Integrin Beta 1/CD29 Rekombinanter Antikörper

Integrin Beta 1/CD29 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

TS2/16

Kat-Nr. : CL594-65601

Synonyme

ITGB1, CD29, FNRB, Glycoprotein IIa, GPIIA

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  FC experiment of human PBMCs using CL594-65601

  1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD29 (TS2/16) Mouse IgG2a RecAb (CL594-65601, Clone: TS2/16) or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4). Cells were co-stained with APC Anti-Human CD45 (HI30) (APC-65109, Clone: HI30). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-65601

  1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD29 (TS2/16) Mouse IgG2a RecAb (CL594-65601, Clone: TS2/16) or CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-65601 bindet in FC Integrin Beta 1/CD29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Berechnetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC020057
• Gene symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.