CD2AP Polyklonaler Antikörper

CD2AP Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32089-1-AP

Synonyme

CMS, CD2-associated protein, CD2 Associated Protein, Cas ligand with multiple SH3 domains, Adapter protein CMS

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  WB analysis using 32089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32089-1-AP (CD2AP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 32089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32089-1-AP (CD2AP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 32089-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cd2ap antibody (32089-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Daudi-Zellen, Jurkat-Zellen, K-562-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32089-1-AP bindet in WB, IF/ICC, ELISA CD2AP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD2AP fusion protein Ag36919
• Vollständiger Name: CD2-associated protein
• Berechnetes Molekulargewicht: 71 kDa，639aa
• Beobachtetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: NM_012120.2
• Gene symbol: CD2AP
• Gene ID (NCBI): 23607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).