CD2AP Rekombinanter Antikörper

CD2AP Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251428G2

Kat-Nr. : 86727-1-RR

Synonyme

CD2 associated protein, CD2-associated protein, Mesenchyme-to-epithelium transition protein with SH3 domains 1, METS 1, Mets1

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  WB analysis using 86727-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86727-1-RR (Cd2ap antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86727-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86727-1-RR against Mouse CD2AP were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Daudi-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86727-1-RR bindet in WB, ELISA CD2AP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD2AP fusion protein Ag0622
• Vollständiger Name: CD2-associated protein
• Berechnetes Molekulargewicht: 637 aa, 70 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC157901
• Gene symbol: Cd2ap
• Gene ID (NCBI): 12488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).