CD3ε Monoklonaler Antikörper

CD3ε Monoklonal Antikörper für FC, IF

Wirt / Isotyp

hamster / IgG

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

CloneNo.

145-2C11

Kat-Nr. : 65060-1-Ig

Synonyme

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e



Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaus-Splenozyten
Erfolgreiche Detektion in FCC57BL/6 mouse splenocytes

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65060-1-Ig bindet in IF, FC CD3ε und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp hamster / IgG
Klonalität Monoklonal
Typ Antikörper
Immunogen H-2Kb-spezifischer muriner cytotoxischer T-Lymphozyten (CTL) -Klon BM10-37
Vollständiger Name CD3 antigen, epsilon polypeptide
GenBank-ZugangsnummerBC098236
Gene symbol Cd3e
Gene ID (NCBI) 12501
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide.
LagerungsbedingungenStore at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protokolle

Produktspezifische Protokolle
IF protocol for CD3ε antibody 65060-1-IgProtokoll herunterladen
FC protocol for CD3ε antibody 65060-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Helena (Verified Customer) (07-22-2022)

Not many t cells to detect but they were observable in this tissue section.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse brain tissue post stroke
CD3ε Antibody Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in mouse brain tissue post stroke (Cat no:65060-1-Ig)