CD3 Rekombinanter Antikörper

CD3 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

OKT3

Kat-Nr. : 65569-1-PBS

Synonyme

CD3E, CD3 epsilon, CD3-epsilon, OKT3, T3E

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  FC experiment of human PBMCs using 65569-1-MR (same clone as 65569-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a RecAb (65569-1-MR, Clone: OKT3) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65569-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65569-1-MR (same clone as 65569-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a RecAb (65569-1-MR, Clone: OKT3) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65569-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65569-1-PBS bindet in FC CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human T cells
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3
• Gene ID (NCBI): 916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).