CD3 Rekombinanter Antikörper

CD3 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

UCHT1

Kat-Nr. : 65570-1-MR

Synonyme

CD3E, CD3 epsilon, CD3-epsilon, T3E, T-cell surface antigen T3/Leu-4 epsilon chain

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  FC experiment of human PBMCs using 65570-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (UCHT1) Mouse IgG2a RecAb (65570-1-MR, Clone: UCHT1) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65570-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (UCHT1) Mouse IgG2a RecAb (65570-1-MR, Clone: UCHT1) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65570-1-MR bindet in FC CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3
• Gene ID (NCBI): 916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).