CD3 Rekombinanter Antikörper

CD3 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242012G2

Kat-Nr. : 84505-4-PBS

Synonyme

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

---

• 

  WB analysis using 84505-4-RR (same clone as 84505-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84505-4-RR (CD3E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84505-4-RR (same clone as 84505-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84505-4-RR (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84505-4-RR (same clone as 84505-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84505-4-RR (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 84505-4-RR (same clone as 84505-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (84505-4-RR, Clone: 242012G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

84505-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3
• Gene ID (NCBI): 916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This antibody is directed against the epsilon chain of human CD3 molecule.