CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

17A2

Kat-Nr. : CL594-65077

Synonyme

Cd3e, 17A2, AI504783, CD3epsilon, CD3ε

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  FC experiment of mouse splenocytes using CL594-65077

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD4, and 0.25 ug CoraLite®594 Anti-Mouse CD3 (17A2) (CL594-65077, Clone: 17A2) or CoraLite®594 Rat IgG2b Isotype Control (LTF-2) (CL594-65211, Clone: LTF-2). Cells were not fixed.

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  FC experiment of mouse splenocytes using CL594-65077

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD3 (17A2) (CL594-65077, Clone: 17A2) (red) or CoraLite®594 Rat IgG2b Isotype Control (LTF-2) (CL594-65211, Clone: LTF-2) (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-65077 bindet in FC CD3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: γδTCR-positive T-T hybridoma D1
• Vollständiger Name: CD3 antigen, epsilon polypeptide
• GenBank-Zugangsnummer: BC098236
• Gene symbol: CD3ε
• Gene ID (NCBI): 12501
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).