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CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

SK7

Kat-Nr. : CL647-65148

Synonyme

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, SK7

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD3 (SK7) (CL647-65148)

    FC experiment of human PBMCs using CL647-65148

    1x10^6 human PBMCs were surface stained with CL405 anti-Human CD19 (CL405-65197) and 5 ul CoraLite® Plus 647 Anti-Human CD3 (CL647-65148, Clone:SK7) or CoraLite® Plus 647 Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD3 (SK7) (CL647-65148)

    FC experiment of human PBMCs using CL647-65148

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD3 (CL647-65148, Clone:SK7) or CoraLite® Plus 647 Mouse IgG1 Isotype Control (CL647-65124, Clone: MOPC-21). Cells were not fixed. Lymphocytes were gated.

Geprüfte Anwendungen

Erfolgreiche Detektion in FChumane PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65148 bindet in FC CD3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen k.A.
Vollständiger Name CD3e molecule, epsilon (CD3-TCR complex)
Berechnetes Molekulargewicht 207 aa, 23 kDa
GenBank-ZugangsnummerBC049847
Gene symbol CD3
Gene ID (NCBI) 916
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
FC protocol for CL Plus 647 CD3 antibody CL647-65148Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Sareena (Verified Customer) (08-09-2023)

Performed well with compensation beads

  • Applications: Flow Cytometry
FH

Sareena (Verified Customer) (07-28-2023)

worked well in duplex stain

  • Applications: Flow Cytometry
FH

Sunil (Verified Customer) (07-28-2023)

Worked great as part of a panel for staining comp beads

  • Applications: Flow Cytometry