CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(4)

CloneNo.

OKT3

Kat-Nr. : FITC-65133

Synonyme

CD3E, CD3 epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, OKT3

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  FC experiment of human PBMCs using FITC-65133

  1x10^6 human PBMCs were surface stained with CL405 Anti-Human CD19 (CL405-65197) and 5ul FITC Plus Anti-Human CD3 (FITC-65133, Clone:OKT3) or FITC Plus Mouse IgG2a Isotype Control (FITC-65244, Clone: MOPC-173). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65133

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 (FITC-65133, Clone:OKT3) or FITC Plus Mouse IgG2a Isotype Control (FITC-65244, Clone: MOPC-173). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 2 publications below

Produktinformation

FITC-65133 bindet in IF, FC CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human T cells
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3
• Gene ID (NCBI): 916
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publikationen

Species	Application	Title
		Int J Pharm; A self-assembly active nanomodulator based on berberine for photothermal immunotherapy of breast cancer via dual regulation of immune suppression; Authors - Jiang Sun
human	FC	J Biol Eng; Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units; Authors - Jyunna Yoshida
	IF	J Med Chem; Platinum(IV) Complexes as Inhibitors of STAT3 and Regulators of the Tumor Microenvironment To Control Breast Cancer; Authors - Linxiang Cai
human	FC	Sci Rep; Construction of IgG-Fab2 bispecific antibody via intein-mediated protein trans-splicing reaction; Authors - Risa Yamada