CD3 Monoklonaler Antikörper

CD3 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen(3)

CloneNo.

OKT3

Rezensionen (1)

Kat-Nr. : PE-65133

Synonyme

CD3, CD3E, T3E, TCRE

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  FC experiment of human PBMCs using PE-65133

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD3 (PE-65133, Clone:OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PE-65133

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD3 (PE-65133, Clone: OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 3 publications below

Produktinformation

PE-65133 bindet in FC CD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human T cells
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3
• Gene ID (NCBI): 916
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publikationen

Species	Application	Title
	FC	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan
human	FC	DNA Cell Biol; Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.; Authors - Yuan-Hao Wu
human	FC	Adv Sci (Weinh); Lipid Nanoparticle Delivery System for mRNA Encoding B7H3-redirected Bispecific Antibody Displays Potent Antitumor Effects on Malignant Tumors; Authors - Cheng Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amelia (Verified Customer) (12-19-2022)

CD3 antibody worked as it should, good separation of positive/negative population.

• Applications: Flow Cytometry