CD3 Rekombinanter Antikörper

CD3 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Kaninchen

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242568D11

Kat-Nr. : 98349-1-PBS

Synonyme

CD3 epsilon + CD3 gamma, CD3 epsilon/CD3 gamma, CD3 epsilon, CD3 gamma, CD3e

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  FC experiment of rabbit splenocytes using 98349-1-MR (same clone as 98349-1-PBS)

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD3 Mouse RecAb (98349-1-MR, Clone:242568D11) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with FITC Anti-Rabbit CD4. Cells were not fixed. This data was developed using the same antibody clone with 98349-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rabbit splenocytes using 98349-1-MR (same clone as 98349-1-PBS)

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD3 Mouse RecAb (98349-1-MR, Clone: 242568D11) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 98349-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98349-1-MR

  Biolayer interferometry (BLl) kinetic assays of 98349-1-MR against Rabbit CD3E were performed. The affinity constant is 17.3 nM.

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Geprüfte Anwendungen

Produktinformation

98349-1-PBS bindet in FC CD3 und zeigt Reaktivität mit Kaninchen

• Getestete Reaktivität: Kaninchen
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD3 fusion protein Eg3525
• Vollständiger Name: T-cell surface glycoprotein CD3 epsilon chain
• Gene symbol: CD3E
• Gene ID (NCBI): 100008616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD3 epsilon(CD3ε) and CD3 gamma (CD3γ) are transmembrane subunits of the CD3 complex, which is essential for the assembly and surface expression of the T cell receptor (TCR). All of the CD3 subunits carry the so-called immunoreceptor tyrosine-based activation motif (ITAM) in the intracytoplasmic region. The signals are initiated by the specific phosphorylation of two tyrosine residues located in the conserved ITAM (PMID: 21269565).