CD30/TNFRSF8 Rekombinanter Antikörper

CD30/TNFRSF8 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241710B9

Kat-Nr. : 84420-7-RR

Synonyme

CD30, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8, Tumor necrosis factor receptor superfamily member 8

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  WB analysis of EL-4 using 84420-7-RR

  EL-4 cells were subjected to SDS PAGE followed by western blot with 84420-7-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84420-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84420-7-RR (CD30/TNFRSF8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EL-4 cells
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84420-7-RR bindet in WB, IHC, ELISA CD30/TNFRSF8 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD30/TNFRSF8 fusion protein Eg1307
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 8
• Berechnetes Molekulargewicht: 53kDa
• Beobachtetes Molekulargewicht: 105-120 kDa
• GenBank-Zugangsnummer: NM_009401.2
• Gene symbol: Tnfrsf8
• Gene ID (NCBI): 21941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).