CD30/TNFRSF8 Rekombinanter Antikörper

CD30/TNFRSF8 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241710B4

Kat-Nr. : 98209-1-RR

Synonyme

241710B4, CD30, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8

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  FC experiment of mouse splenocytes using 98209-1-RR

  1x10^6 anti-CD3/CD28-treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30/TNFRSF8 Rabbit RecAb (98209-1-RR, Clone: 241710B4) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD30/TNFRSF8 Rabbit Recombinant Antibody (98209-1-RR, Clone: 241710B4) and PE-Conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98209-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98209-1-RR against Mouse CD30/TNFRSF8 were performed. The affinity constant is 1.64 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98209-1-RR bindet in FC CD30/TNFRSF8 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD30/TNFRSF8 fusion protein Eg1307
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 8
• Berechnetes Molekulargewicht: 53kDa
• GenBank-Zugangsnummer: NM_009401.2
• Gene symbol: Tnfrsf8
• Gene ID (NCBI): 21941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).