CD300e Rekombinanter Antikörper

CD300e Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242031C10

Kat-Nr. : 98235-1-RR

Synonyme

CMRF35A5, CMRF35 A5, CLM2, CLM 2, CD300LE

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  FC experiment of human PBMCs using 98235-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD300e Rabbit Recombinant Antibody (98235-1-RR, Clone: 242031C10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed

• 

  FC experiment of human PBMCs using 98235-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD300e Rabbit Recombinant Antibody (98235-1-RR, Clone: 242031C10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98235-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98235-1-RR against Human CD300e were performed. The affinity constant is 0.456 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98235-1-RR bindet in FC CD300e und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD300e molecule
• Berechnetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: NM_181449.2
• Gene symbol: CD300E
• Gene ID (NCBI): 342510
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD300e, also known as immune receptor expressed on myeloid cells 2 (IREM-2), CLM-2, or CMRF35-A5, is one of the CD300 family members which belong to the immunoglobulin (Ig) superfamily (PMID: 15557162; 20039296). CD300e is a type I transmembrane glycoprotein that contains an extracellular region comprising an Ig-like domain, a transmembrane region, and a short cytoplasmic tail (PMID: 15557162; 29358324). It is by monocytes and myeloid dendritic cells and transmits an immune-activating signal by interacting with DNAX-activating protein 12 (DAP12) (PMID: 29358324).