CD302 Rekombinanter Antikörper

CD302 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242893D7

Kat-Nr. : 85813-5-PBS

Synonyme

CD302 antigen, Clec13a, C-type lectin domain family 13 member A, Type I transmembrane C-type lectin receptor DCL-1

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  WB analysis of rat liver using 85813-5-RR (same clone as 85813-5-PBS)

  Rat liver tissue was subjected to SDS PAGE, followed by western blot with 85813-5-RR (Cd302 antibody) at a dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

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  WB analysis using 85813-5-RR (same clone as 85813-5-PBS)

  Mouse liver tissue was subjected to SDS PAGE followed by western blot with 85813-5-RR (Cd302 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85813-5-RR (same clone as 85813-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 85813-5-RR (same clone as 85813-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 85813-5-RR (same clone as 85813-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 85813-5-RR (same clone as 85813-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85813-5-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85813-5-PBS bindet in WB, IHC, Indirect ELISA CD302 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD302 fusion protein Eg2660
• Vollständiger Name: CD302 antigen
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_025422.4
• Gene symbol: Cd302
• Gene ID (NCBI): 66205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis. In mice and humans, CD302 is primarily expressed in the liver, lungs, lymph nodes (LNs), spleen, and bone marrow. In CD302 gene knockout (CD302KO) mice, the migration frequency and number of myeloid dendritic cells (mDC) in CD302KO lymph nodes were reduced compared to the wild type, confirming the functional role of CD302 in mDC migration.