CD302 Rekombinanter Antikörper

CD302 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242893D7

Kat-Nr. : 85813-5-RR

Synonyme

CD302 antigen, Clec13a, C-type lectin domain family 13 member A, Type I transmembrane C-type lectin receptor DCL-1

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  WB analysis of rat liver using 85813-5-RR

  Rat liver tissue was subjected to SDS PAGE, followed by western blot with 85813-5-RR (Cd302 antibody) at a dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85813-5-RR

  Mouse liver tissue was subjected to SDS PAGE followed by western blot with 85813-5-RR (Cd302 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 85813-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 85813-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 85813-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 85813-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85813-5-RR (CD302 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenlebergewebe, Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85813-5-RR bindet in WB, IHC, ELISA CD302 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD302 fusion protein Eg2660
• Vollständiger Name: CD302 antigen
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_025422.4
• Gene symbol: Cd302
• Gene ID (NCBI): 66205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD302 is a C-type lectin receptor involved in cell adhesion and migration, as well as endocytosis and phagocytosis. In mice and humans, CD302 is primarily expressed in the liver, lungs, lymph nodes (LNs), spleen, and bone marrow. In CD302 gene knockout (CD302KO) mice, the migration frequency and number of myeloid dendritic cells (mDC) in CD302KO lymph nodes were reduced compared to the wild type, confirming the functional role of CD302 in mDC migration.