CD31 Rekombinanter Antikörper

CD31 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242041E12

Kat-Nr. : 84607-4-RR

Synonyme

242041E12, Pecam, PECAM 1, Pecam1, PECAM-1

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  WB analysis using 84607-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84607-4-RR (CD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 84607-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84607-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of bEnd.3 using 84607-4-RR

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31/PECAM-1 antibody (84607-4-RR, Clone: 242041E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84607-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84607-4-RR against Mouse CD31 were performed. The affinity constant is 0.882 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bEnd.3-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Maushirngewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: bEnd.3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84607-4-RR bindet in WB, IHC, IF/ICC, ELISA CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD31 fusion protein Eg0846
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• Berechnetes Molekulargewicht: 81 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: NM_001305157.1
• Gene symbol: CD31
• Gene ID (NCBI): 18613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).