CD31 Rekombinanter Antikörper

CD31 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242041B5

Kat-Nr. : 98258-1-PBS

Synonyme

242041B5, Pecam, PECAM 1, Pecam1, PECAM-1

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  FC experiment of mouse splenocytes using 98258-1-RR (same clone as 98258-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit RecAb (98258-1-RR, Clone: 242041B5) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed. This data was developed using the same antibody clone with 98258-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98258-1-RR (same clone as 98258-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit RecAb (98258-1-RR, Clone: 242041B5) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98258-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98258-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98258-1-RR against Mouse CD31 were performed. The affinity constant is 0.547 nM.

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Geprüfte Anwendungen

Produktinformation

98258-1-PBS bindet in FC CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD31 fusion protein Eg0846
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• Berechnetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: NM_001305157.1
• Gene symbol: CD31
• Gene ID (NCBI): 18613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).