CD31 Rekombinanter Antikörper

CD31 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242041B5

Kat-Nr. : 98258-1-RR

Synonyme

242041B5, Pecam, PECAM 1, Pecam1, PECAM-1

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  FC experiment of mouse splenocytes using 98258-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit RecAb (98258-1-RR, Clone: 242041B5) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD45R (B220). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98258-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.15 ug Anti-Mouse CD31 Rabbit RecAb (98258-1-RR, Clone: 242041B5) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98258-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98258-1-RR against Mouse CD31 were performed. The affinity constant is 0.547 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98258-1-RR bindet in FC CD31 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD31 fusion protein Eg0846
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• Berechnetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: NM_001305157.1
• Gene symbol: CD31
• Gene ID (NCBI): 18613
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).