CD31 Rekombinanter Antikörper

CD31 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250372H6

Kat-Nr. : 98457-1-PBS

Synonyme

Pecam, Pecam1, PECAM-1, Platelet endothelial cell adhesion molecule

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  FC experiment of rat splenocytes using 98457-1-RR (same clone as 98457-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD31 Rabbit RecAb (98457-1-RR, Clone: 250372H6) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody (CL647-65578, Clone: OX-33). Cells were not fixed. This data was developed using the same antibody clone with 98457-1-PBS in a different storage buffer formulation.

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  FC experiment of rat splenocytes using 98457-1-RR (same clone as 98457-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD31 Rabbit RecAb (98457-1-RR, Clone: 250372H6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98457-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98457-1-PBS bindet in FC CD31 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD31 fusion protein Eg1456
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• Berechnetes Molekulargewicht: 76kDa
• GenBank-Zugangsnummer: NP_113779.1
• Gene symbol: Pecam1
• Gene ID (NCBI): 29583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).