CD31 Polyklonaler Antikörper

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• KD/KO Validated

CD31 Polyklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

Kat-Nr. : CL488-11265

Synonyme

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

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  IF Staining of human tonsillitis using CL488-11265

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-11265) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using CL488-11265

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-11265) at dilution of 1:200.

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  IF Staining of HUVEC using CL488-11265

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite® Plus 488 CD31 antibody (CL488-11265) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

CL488-11265 bindet in IF/ICC, IF-P CD31 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD31 fusion protein Ag1787
• Vollständiger Name: platelet/endothelial cell adhesion molecule
• Berechnetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC022512
• Gene symbol: CD31
• Gene ID (NCBI): 5175
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publikationen

Species	Application	Title
human	IF	Biochem Biophys Res Commun; Dulaglutide restores endothelial progenitor cell levels in diabetic mice and mitigates high glucose-induced endothelial injury through SIRT1-mediated mitochondrial fission; Authors - Xi Mei
human	IF	FASEB J; CD74+ fibroblasts proliferate upon mechanical stretching to promote angiogenesis in keloids; Authors - Jingheng Zhang