CD31 Monoklonaler Antikörper

CD31 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF-P

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

3F8E2

Kat-Nr. : CL594-66065

Synonyme

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

---

• 

  IF Staining of human tonsillitis using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

• 

  IF Staining of human placenta using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 3F8E2 ) at dilution of 1:200.

• 

  IF Staining of human placenta using CL594-66065

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 3F8E2 ) at dilution of 1:200.

• 

  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in teal) and Phalloidin (in orange). DAPI in gray. Image credit: Shuntaro Yamada, PhD,

• 

  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in blue) and Phalloidin (in green). DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

• 

  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in purple) and Phalloidin (in teal). DAPI in yellow. Image credit: Shuntaro Yamada, PhD,

• 

  IF Staining of human umbilical cord endothelial cells using CL594-66065

  Immunofluorescent analysis of fixed human umbilical cord endothelial cells using CoraLite 594 CD31 antibody (CL594-66065, in yellow) and Phalloidin (in green). DAPI in red. Image credit: Shuntaro Yamada, PhD, University of Bergen (IG: @stemcell_art)

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe, humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

CL594-66065 bindet in WB, IF-P CD31 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD31 fusion protein Ag1787
• Vollständiger Name: platelet/endothelial cell adhesion molecule
• Berechnetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC022512
• Gene symbol: CD31
• Gene ID (NCBI): 5175
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publikationen

Species	Application	Title
human	WB	Cell Metab; Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity; Authors - Zilong Zhang