CD31/PECAM-1 Rekombinanter Antikörper

CD31/PECAM-1 Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

251101A10

Kat-Nr. : 80773-6-PBS

Synonyme

CD31, EndoCAM, GPIIA, GPIIA', PECA1

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  Sandwich ELISA standard curve of MP02437-2

  Sandwich ELISA standard curve of MP02437-2, Human CD31 Recombinant Matched Antibody Pair - PBS only. 80773-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4658. 80773-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP02437-2

  Plasma of six individual healthy human donors was measured. The human CD31 concentration of detected samples was determined to be 18.66 ng/mL with a range of 9.29 - 51.94 ng/mL.

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  Sample test of MP02437-2

  Urine of six individual healthy human donors was measured. The human CD31 concentration of detected samples was determined to be 0.47 ng/mL with a range of 0.05 - 1.39 ng/mL.

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  Sample test of MP02437-2

  THP-1 cells (3 x 10^6 cells/mL) were cultured in RPMI with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured unstimulated or stimulated with 1 ug/mL LPS for 1 day. Aliquots of the cell culture supernatants were removed, assayed for levels of CD31, measured 0.47 ng/mL and 0.74 ng/mL respectively.

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Geprüfte Anwendungen

Produktinformation

80773-6-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test CD31/PECAM-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD31/PECAM-1 fusion protein Eg4658
• Vollständiger Name: platelet/endothelial cell adhesion molecule
• Berechnetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: NM_000442.5
• Gene symbol: CD31
• Gene ID (NCBI): 5175
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.