CD31 Rekombinanter Antikörper

CD31 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250372F8

Kat-Nr. : 85898-4-PBS

Synonyme

Pecam, Pecam1, PECAM-1, Platelet endothelial cell adhesion molecule

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  WB analysis using 85898-4-RR (same clone as 85898-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 85898-4-RR (same clone as 85898-4-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85898-4-RR (CD31/PECAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 85898-4-RR (same clone as 85898-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 85898-4-RR (CD31 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 85898-4-RR (same clone as 85898-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 85898-4-RR (CD31 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 85898-4-RR (same clone as 85898-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD31 antibody (85898-4-RR, Clone: 250372F8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85898-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85898-4-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD31 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD31 fusion protein Eg1456
• Vollständiger Name: platelet/endothelial cell adhesion molecule 1
• Berechnetes Molekulargewicht: 76kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: NP_113779.1
• Gene symbol: Pecam1
• Gene ID (NCBI): 29583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).