CD314/NKG2D Monoklonaler Antikörper

CD314/NKG2D Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

1D11

Kat-Nr. : APC-65188

Synonyme

CD314, KLRK1, 1D11, D12S2489E, Killer cell lectin-like receptor subfamily K member 1

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  FC experiment of human PBMCs using APC-65188

  1X10^6 human PBMCs were surface co-stained with CL488 Anti-Human CD8a (CL488-65204, Clone: UCHT4) and 10 ul APC Anti-Human CD314/NKG2D (APC-65188, Clone:1D11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using APC-65188

  1X10^6 human PBMCs were surface stained with 10 ul APC Anti-Human CD314/NKG2D (APC-65188, Clone:1D11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65188 bindet in FC CD314/NKG2D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NKL-Zelllinie
• Vollständiger Name: killer cell lectin-like receptor subfamily K, member 1
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC039836
• Gene symbol: KLRK1
• Gene ID (NCBI): 22914
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit Allophycocyanin (APC) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).