CD314/NKG2D Monoklonaler Antikörper

CD314/NKG2D Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

1D11

Kat-Nr. : PE-65188

Synonyme

CD314, KLRK1, 1D11, D12S2489E, Killer cell lectin-like receptor subfamily K member 1

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  FC experiment of human peripheral blood lymphocytes using PE-65188

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD8 (APC-65146, Clone: SK1) and 10 ul PE Anti-Human CD314/NKG2D (PE-65188, Clone: 1D11). Cells were not fixed.

• 

  FC experiment of human peripheral blood lymphocytes using PE-65188

  1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD8 (APC-65146, Clone: SK1) and PE-Mouse IgG1 isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane Lymphozyten aus peripherem Blut

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65188 bindet in FC CD314/NKG2D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NKL-Zelllinie
• Vollständiger Name: killer cell lectin-like receptor subfamily K, member 1
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC039836
• Gene symbol: KLRK1
• Gene ID (NCBI): 22914
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).