CD320 Polyklonaler Antikörper

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• KD/KO Validated

CD320 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 10343-1-AP

Synonyme

8D6, 8D6A, FDC-signaling molecule 8D6, FDC-SM-8D6, TCblR

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  WB analysis using 10343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10343-1-AP (CD320 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 10343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10343-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, humanes Plazenta-Gewebe, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Produktinformation

10343-1-AP bindet in WB, IHC, IF, ELISA CD320 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD320 fusion protein Ag0344
• Vollständiger Name: CD320 molecule
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa, 35-40 kDa
• GenBank-Zugangsnummer: BC000668
• Gene symbol: CD320
• Gene ID (NCBI): 51293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 35-40 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

Publikationen

Species	Application	Title
cat,canine	IHC	Oncotarget; Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.; Authors - Annette M Sysel
mouse	IF	Int J Biol Macromol; CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.; Authors - Yue Chen
human	WB,IF	FASEB J; Identification of a novel mechanism for meso-tetra (4-carboxyphenyl) porphyrin (TCPP) uptake in cancer cells.; Authors - David J Elzi ; KD Validated
human	IHC	Anticancer Res; Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.; Authors - Sysel Annette M AM
human	IHC	Heliyon; Secreted proteins in plasma and placenta as novel non-invasive biomarkers for intrahepatic cholestasis of pregnancy: A case-control study; Authors - Lingyan Chen
human	IF	Cell Rep; FTY720 requires vitamin B12-TCN2-CD320 signaling in astrocytes to reduce disease in an animal model of multiple sclerosis; Authors - Deepa Jonnalagadda

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joseph (Verified Customer) (09-17-2020)

We evaluated several CD320 antibodies but the Proteintech sequence provided better specificity and greater sensitivity to the CD320 protein target.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50 dilution human; 1:50 dilution canine; 1:25 dilution feline
• Cell Tissue Type: human, canine and feline malignant various

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