CD320 Polyklonaler Antikörper

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CD320 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10343-1-PBS

Synonyme

8D6, 8D6A, FDC-signaling molecule 8D6, FDC-SM-8D6, TCblR

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  WB analysis using 10343-1-AP (same clone as 10343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10343-1-AP (CD320 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 10343-1-AP (same clone as 10343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 10343-1-AP (same clone as 10343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10343-1-PBS bindet in WB, IHC, Indirect ELISA CD320 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD320 fusion protein Ag0344
• Vollständiger Name: CD320 molecule
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa, 35-40 kDa
• GenBank-Zugangsnummer: BC000668
• Gene symbol: CD320
• Gene ID (NCBI): 51293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 35-40 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

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