E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240159G8

Kat-Nr. : 98030-1-PBS

Synonyme

CD324 (E-cadherin), 240159G8, Arc 1, Cadherin 1, Cadherin-1

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  FC experiment of MCF-7 using 98030-1-RR (same clone as 98030-1-PBS)

  1x10^6 MCF-7 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone: 240159G8) (red) or Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98030-1-PBS in a different storage buffer formulation.

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  FC experiment of HT-29 using 98030-1-RR (same clone as 98030-1-PBS)

  1x10^6 HT-29 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) (red) or Isotype Control (blue), and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98030-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98030-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98030-1-RR against Human E-cadherin were performed. The affinity constant is 37.3 pM.

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Geprüfte Anwendungen

Produktinformation

98030-1-PBS bindet in FC E-cadherin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Ag14973
• Vollständiger Name: cadherin 1, type 1, E-cadherin (epithelial)
• Berechnetes Molekulargewicht: 882 aa, 97 kDa
• GenBank-Zugangsnummer: BC141838
• Gene symbol: E-cadherin
• Gene ID (NCBI): 999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.