E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242018A7

Kat-Nr. : 98323-1-RR

Synonyme

242018A7, Cadherin-1, CD324, Cdh1, E-Cad/CTF1

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  FC experiment of PC-12 using 98323-1-RR

  1x10^6 PC-12 cells were surface stained with 0.25 ug Anti-Rat E-cadherin Rabbit RecAb (98323-1-RR, Clone: 242018A7) (red),or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue)and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PC-12-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98323-1-RR bindet in FC E-cadherin und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: E-cadherin fusion protein Eg1229
• Vollständiger Name: cadherin 1
• Berechnetes Molekulargewicht: 99 kDa
• GenBank-Zugangsnummer: NM_031334.1
• Gene symbol: Cdh1
• Gene ID (NCBI): 83502
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.