CD324 (E-cadherin) Rekombinanter Antikörper

CD324 (E-cadherin) Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240159B4

Kat-Nr. : CL594-98030-2

Synonyme

E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1



Geprüfte Anwendungen

Erfolgreiche Detektion in FCHT-29-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98030-2 bindet in FC CD324 (E-cadherin) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD324 (E-cadherin) fusion protein Ag14973
Vollständiger Name cadherin 1, type 1, E-cadherin (epithelial)
Berechnetes Molekulargewicht 882 aa, 97 kDa
GenBank-ZugangsnummerBC141838
Gene symbol E-cadherin
Gene ID (NCBI) 999
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
FC protocol for CL594 CD324 (E-cadherin) antibody CL594-98030-2Download protocol
STANDARD-PROTOKOLLE
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