E-cadherin Rekombinanter Antikörper

E-cadherin Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240159G8

Kat-Nr. : CL594-98030

Synonyme

CD324 (E-cadherin), 240159G8, Arc 1, Cadherin 1, Cadherin-1



Geprüfte Anwendungen

Erfolgreiche Detektion in FCMCF-7-Zellen, HT-29-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98030 bindet in FC E-cadherin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen E-cadherin fusion protein Ag14973
Vollständiger Name cadherin 1, type 1, E-cadherin (epithelial)
Berechnetes Molekulargewicht 882 aa, 97 kDa
GenBank-ZugangsnummerBC141838
Gene symbol E-cadherin
Gene ID (NCBI) 999
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
FC protocol for CL594 E-cadherin antibody CL594-98030Download protocol
STANDARD-PROTOKOLLE
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