E-cadherin Monoklonaler Antikörper

E-cadherin Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

human, Hund, Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

CloneNo.

DECMA-1

Kat-Nr. : CL647-65241

Synonyme

CD324 (E-cadherin), cadherin 1, Cdh1, Ecad, L CAM

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  FC experiment of MDCK using CL647-65241

  1X10^6 MDCK cells were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD324 (E-cadherin) (CL647-65241, Clone:DECMA-1) (red), or or 0.5 ug CoraLite® Plus 647 Rat IgG1 Isotype Control (HRPN) (CL647-65212, Clone: HRPN) (blue). Cells were not fixed.

• 

  FC experiment of MDCK using CL647-65241

  1X10^6 MDCK cells were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD324 (E-cadherin) (CL647-65241, Clone:DECMA-1), or 0.5 ug CoraLite® Plus 647 Rat IgG1 Isotype Control (HRPN) (CL647-65212, Clone: HRPN). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: MDCK-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65241 bindet in FC E-cadherin und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Mouse embryonal carcinoma cell line PCC4 Aza RI
• Vollständiger Name: cadherin 1
• GenBank-Zugangsnummer: BC098501
• Gene symbol: E-cadherin
• Gene ID (NCBI): 12550
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: N/A
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Publikationen

Species	Application	Title
mouse		Nat Commun; Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition; Authors - Minglu Zhou