CD33 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

CD33 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241711D2

Kat-Nr. : 84341-3-PBS

Synonyme

241711D2, CD33 molecule, gp67, p67, Sialic acid-binding Ig-like lectin 3

---

• 

  WB analysis of THP-1 using 84341-3-RR (same clone as 84341-3-PBS)

  WB result of CD33 antibody (84341-3-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD33 transfected THP-1 cells. This data was developed using the same antibody clone with 84341-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 84341-3-RR (same clone as 84341-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84341-3-RR (CD33 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84341-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84341-3-RR (same clone as 84341-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-3-RR (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84341-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84341-3-RR (same clone as 84341-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-3-RR (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84341-3-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

84341-3-PBS bindet in WB, IHC, Indirect ELISA CD33 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD33 fusion protein Eg2138
• Vollständiger Name: CD33 molecule
• Berechnetes Molekulargewicht: 364 aa, 40 kDa
• Beobachtetes Molekulargewicht: 55-75 kDa
• GenBank-Zugangsnummer: BC028152
• Gene symbol: CD33
• Gene ID (NCBI): 945
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.