CD34 Monoklonaler Antikörper

CD34 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

CloneNo.

3C8G12

Rezensionen (1)

Kat-Nr. : 60180-1-Ig

Synonyme

3C8G12, CD34 molecule

---

• 

  WB analysis of human placenta using 60180-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human uterus using 60180-1-Ig

  human uterus tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 60180-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 60180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 60180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 60180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60180-1-Ig (CD34 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60180-1-Ig (CD34 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 60180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 60180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver using 60180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, magenta), ASGR1 antibody (11739-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA502, orange), CoraLite® Plus 488 CD163 antibody (CL488-16646, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Hodengewebe, humanes Uterusgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe, humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 13 publications below
• IF: See 8 publications below

Produktinformation

60180-1-Ig bindet in WB, IHC, IF-P, ELISA CD34 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD34 fusion protein Ag5996
• Vollständiger Name: CD34 molecule
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC039146
• Gene symbol: CD34
• Gene ID (NCBI): 947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).

Publikationen

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	IF	Front Immunol; Thrombotic microangiopathy mediates poor prognosis among lupus nephritis via complement lectin and alternative pathway activation; Authors - Binshan Zhang
	IHC	Front Bioeng Biotechnol; Primary Repair for Treating Acute Proximal Anterior Cruciate Ligament Tears: A Histological Analysis and Prospective Clinical Trial.; Authors - Yue Yang
human		Exp Cell Res; Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch.; Authors - Xun Xi
	IHC	Biomed Mater; The additive effects of photobiomodulation and bioactive glasses on enhancing early angiogenesis.; Authors - Lidong Huang
human	IHC	Onco Targets Ther; Expression and significance of GRHL2 in esophageal cancer.; Authors - Chengcheng Shao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Reyes (Verified Customer) (09-24-2025)

CD34 (in green) failed to mark specifically the blood vessels of the FFPE human brain cortex tissue. It seemed to mark some blood vessels, missing others. But it marked highly the background of the tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

CD34 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:60180-1-Ig)