CD34 Monoklonaler Antikörper

CD34 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

4F11H3

Kat-Nr. : 60180-2-Ig

Synonyme

CD34 molecule, 4F11H3

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  WB analysis of human placenta using 60180-2-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human uterus using 60180-2-Ig

  human uterus tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 60180-2-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ovary cancer using 60180-2-Ig

  human ovary cancer cells were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60180-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humane Ovarialkarzinomzellen, humanes Hodengewebe, humanes Uterusgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

60180-2-Ig bindet in WB, IHC, IF, ELISA CD34 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD34 fusion protein Ag5996
• Vollständiger Name: CD34 molecule
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC039146
• Gene symbol: CD34
• Gene ID (NCBI): 947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).

Publikationen

Species	Application	Title
human	IHC	Front Oncol; Prognostic Impact of the Angiogenic Gene POSTN and Its Related Genes on Lung Adenocarcinoma.; Authors - Dongfeng Sun
		BMC Cancer; Novel fusion protein PK5-RL-Gal-3C inhibits hepatocellular carcinoma via anti-angiogenesis and cytotoxicity; Authors - Xiaoge Gao
human	IF	STAR Protoc; Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature; Authors - Léa Pechtimaldjian