CD34 Monoklonaler Antikörper

CD34 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4F11H3

Kat-Nr. : 60180-2-PBS

Synonyme

CD34 molecule, 4F11H3

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  WB analysis of human placenta using 60180-2-Ig (same clone as 60180-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human uterus using 60180-2-Ig (same clone as 60180-2-PBS)

  human uterus tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 60180-2-Ig (same clone as 60180-2-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human ovary cancer using 60180-2-Ig (same clone as 60180-2-PBS)

  human ovary cancer cells were subjected to SDS PAGE followed by western blot with 60180-2-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60180-2-Ig (same clone as 60180-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60180-2-Ig (same clone as 60180-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60180-2-Ig (same clone as 60180-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60180-2-Ig (same clone as 60180-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60180-2-Ig (same clone as 60180-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60180-2-Ig (same clone as 60180-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60180-2-Ig (same clone as 60180-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-2-Ig (CD34 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60180-2-PBS bindet in WB, IHC, Indirect ELISA CD34 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD34 fusion protein Ag5996
• Vollständiger Name: CD34 molecule
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC039146
• Gene symbol: CD34
• Gene ID (NCBI): 947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).