CD34 Rekombinanter Antikörper

CD34 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240659C7

Kat-Nr. : 83713-2-PBS

Synonyme

240659C7, CD34 antigen

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  WB analysis using 83713-2-RR (same clone as 83713-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83713-2-RR (CD34 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 83713-2-RR (same clone as 83713-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83713-2-RR (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 83713-2-RR (same clone as 83713-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD34 antibody (83713-2-RR, Clone: 240659C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83713-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83713-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83713-2-RR against Mouse CD34 were performed. The affinity constant is 1.09 nM.

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Geprüfte Anwendungen

Produktinformation

83713-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD34 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD34 fusion protein Eg0854
• Vollständiger Name: CD34 antigen
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: NM_133654
• Gene symbol: CD34
• Gene ID (NCBI): 12490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.