CD34 Rekombinanter Antikörper

CD34 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

240659B11

Kat-Nr. : 98069-1-RR

Synonyme

CD34 antigen, 240659B11

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• 

  FC experiment of C57BL/6 mouse bone marrow cells using 98069-1-RR

  1x10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD34 Rabbit Recombinant Antibody (98069-1-RR, Clone:240659B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control . Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98069-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98069-1-RR against Mouse CD34 were performed. The affinity constant is 3.45 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: C57BL/6 mouse bone marrow cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

98069-1-RR bindet in FC CD34 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD34 antigen
• Berechnetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: NM_133654
• Gene symbol: CD34
• Gene ID (NCBI): 12490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Publikationen

Species	Application	Title
rat	FC	Mater Today Bio; Exosome-loaded tannic acid-thioctic acid hydrogel enhances wound healing in coagulation disorders; Authors - Yuesheng Tu