CD34 Monoklonaler Antikörper

CD34 Monoklonal Antikörper für IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF-P

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3C8G12

Kat-Nr. : CL594-60180

Synonyme

CD34, CD34 molecule

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  IF Staining of human liver cancer using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:100, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta), CoraLite® Plus 488 CD163 antibody (CL488-16646, green).

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  IF Staining of human placenta using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®594 CD34 antibody (CL594-60180, Clone: 3C8G12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-60180 (CD34 antibody), at dilution of 1:100.

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  IF Staining of human tonsillitis using CL594-60180

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite®594-conjugated version of this antibody, CL594-60180 (CD34 antibody), at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60180 bindet in IF-P CD34 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD34 fusion protein Ag5996
• Vollständiger Name: CD34 molecule
• Berechnetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC039146
• Gene symbol: CD34
• Gene ID (NCBI): 947
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.