CD35 Rekombinanter Antikörper

CD35 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

E11

Kat-Nr. : 65622-1-MR

Synonyme

KN, E11, CR1, Complement receptor type 1, C3BR

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  FC experiment of human PBMCs using 65622-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65622-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD35 (E11) Mouse IgG2a Recombinant Antibody (65622-1-MR, Clone: E11) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

65622-1-MR bindet in FC CD35 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human CD35 from Acute monocytic leukemia cells and normal blood monocytes
• Vollständiger Name: complement component (3b/4b) receptor 1 (Knops blood group)
• Berechnetes Molekulargewicht: 224 kDa
• GenBank-Zugangsnummer: NM_000573
• Gene symbol: CR1
• Gene ID (NCBI): 1378
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Publikationen

Species	Application	Title
rat	FC	Mater Today Bio; Exosome-loaded tannic acid-thioctic acid hydrogel enhances wound healing in coagulation disorders; Authors - Yuesheng Tu