GITR/TNFRSF18 Monoklonaler Antikörper

GITR/TNFRSF18 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

FITC Fluorescent Dye

Publikationen(1)

CloneNo.

DTA-1

Kat-Nr. : FITC-65102

Synonyme

CD357, GITR, Tnfrsf18, AITR, Glucocorticoid-induced TNFR-related protein

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  FC experiment of mouse splenocytes using FITC-65102

  1X10^6 mouse splenocytes were surface stained with 0.125 ug PE-Anti-Mouse CD4 (PE-65104, clone GK1.5), 0.125 ug APC-Anti-Mouse CD25 (APC-65137, clone PC61.5) and 0.06 ug FITC-Anti-Mouse CD357 (GITR) (FITC-65102, clone DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

FITC-65102 bindet in IF, FC GITR/TNFRSF18 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-CD25+-CD4+-T-Zellen
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 18
• GenBank-Zugangsnummer: BC146517
• Gene symbol: CD357
• Gene ID (NCBI): 21936
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.1% gelatin
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).

Publikationen

Species	Application	Title
mouse	IF	Adv Healthc Mater; Engineered Hollow Nanocomplex Combining Photothermal and Antioxidant Strategies for Targeted Tregs Depletion and Potent Immune Activation in Tumor Immunotherapy; Authors - Qi Sun