CD36 Monoklonaler Antikörper

CD36 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A8C5

Kat-Nr. : 66395-1-PBS

Synonyme

GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb

---

• 

  WB analysis of human heart using 66395-1-Ig (same clone as 66395-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human milk using 66395-1-Ig (same clone as 66395-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66395-1-Ig (same clone as 66395-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66395-1-Ig (CD36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66395-1-Ig (CD36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66395-1-Ig (same clone as 66395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66395-1-Ig (CD36 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66395-1-Ig (same clone as 66395-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66395-1-Ig (CD36 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66395-1-Ig (same clone as 66395-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD36 antibody (66395-1-Ig, Clone: 1A8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 66395-1-Ig (same clone as 66395-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD36 antibody (66395-1-Ig, Clone: 1A8C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66395-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA CD36 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD36 fusion protein Ag13541
• Vollständiger Name: CD36 molecule (thrombospondin receptor)
• Berechnetes Molekulargewicht: 472 aa, 53 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC008406
• Gene symbol: CD36
• Gene ID (NCBI): 948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease

• Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

• Polymorphisms in CD36 can increase susceptibility to malaria.

• AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

• Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.