CD36 Rekombinanter Antikörper

CD36 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Biotin

CloneNo.

241262D5

Kat-Nr. : Biotin-98132

Synonyme

GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb

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  FC experiment of human PBMCs using Biotin-98132

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD36 Rabbit Recombinant Antibody (Biotin-98132, Clone: 241262D5) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human HLA-DR. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-98132

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD36 Rabbit Recombinant Antibody (Biotin-98132, Clone: 241262D5) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-98132 bindet in FC CD36 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD36 molecule (thrombospondin receptor)
• Berechnetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: NM_001001548.3
• Gene symbol: CD36
• Gene ID (NCBI): 948
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease

• Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

• Polymorphisms in CD36 can increase susceptibility to malaria.

• AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

• Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.