CD37 Rekombinanter Antikörper

CD37 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250262A3

Kat-Nr. : 85917-1-PBS

Synonyme

Leukocyte antigen CD37, Tetraspanin 26, TSPAN26

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  WB analysis using 85917-1-RR (same clone as 85917-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85917-1-RR (Cd37 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85917-1-RR (same clone as 85917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85917-1-RR (CD37 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 85917-1-RR (same clone as 85917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85917-1-RR (CD37 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 85917-1-RR (same clone as 85917-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IF Staining of rat spleen using 85917-1-RR (same clone as 85917-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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  IF Staining of A20 using 85917-1-RR (same clone as 85917-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A20 cells using Cd37 antibody (85917-1-RR, Clone: 250262A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85917-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

85917-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA CD37 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD37 fusion protein Eg2729
• Vollständiger Name: CD37 antigen
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_007645.4
• Gene symbol: Cd37
• Gene ID (NCBI): 12493
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).