CD38 Rekombinanter Antikörper

CD38 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241246B1

Kat-Nr. : 84070-2-PBS

Synonyme

241246B1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP ribosyl cyclase 1

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  WB analysis using 84070-2-RR (same clone as 84070-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84070-2-RR (CD38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  WB analysis using 84070-2-RR (same clone as 84070-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84070-2-RR (CD38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00991-2

  Sandwich ELISA standard curve of MP00991-2, Human CD38 Recombinant Matched Antibody Pair - PBS only. 84070-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0110. 84070-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00991-2

  Serum of six individual healthy human donors was measured. The CD38 concentration of detected samples was determined to be 0.3 ng/mL with a range of ND-1.0 ng/mL.

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  Sample test of MP00991-2

  The mean CD38 concentration was determined to be 244.5 ng/mL in Daudi cell extract based on a 1.8 mg/mL extract load and 65.6 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load.

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  IF Staining of Raji using 84070-2-RR (same clone as 84070-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (84070-2-RR, Clone: 241246B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 84070-2-RR (same clone as 84070-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (84070-2-RR, Clone: 241246B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00991-2

  Cytometric bead array standard curve of MP00991-2, CD38 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84070-1-PBS. Detection antibody: 84070-2-PBS. Standard: Eg0110. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84070-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84070-2-RR against Human CD38 were performed. The affinity constant is 0.999 nM.

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Geprüfte Anwendungen

Produktinformation

84070-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CD38 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD38 fusion protein Eg0110
• Vollständiger Name: CD38 molecule
• Berechnetes Molekulargewicht: 300 aa, 34 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC007964
• Gene symbol: CD38
• Gene ID (NCBI): 952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).