CD38 Rekombinanter Antikörper

CD38 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241246D5

Kat-Nr. : 84070-6-PBS

Synonyme

ADPRC 1, ADP ribosyl cyclase 1, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase

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  WB analysis using 84070-6-RR (same clone as 84070-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84070-6-RR (CD38 antibody) at dilution of 1:17000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84070-6-RR (same clone as 84070-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84070-6-RR (same clone as 84070-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84070-6-RR (same clone as 84070-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 84070-6-RR (same clone as 84070-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (84070-6-RR, Clone: 241246D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84070-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84070-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84070-6-RR against Human CD38 were performed. The affinity constant is 1.11 nM.

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Geprüfte Anwendungen

Produktinformation

84070-6-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD38 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD38 molecule
• Berechnetes Molekulargewicht: 300 aa, 34 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC007964
• Gene symbol: CD38
• Gene ID (NCBI): 952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).