CD38 Rekombinanter Antikörper

CD38 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241246D5

Kat-Nr. : 84070-6-RR

Synonyme

ADPRC 1, ADP ribosyl cyclase 1, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase

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  WB analysis using 84070-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84070-6-RR (CD38 antibody) at dilution of 1:17000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 84070-6-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84070-6-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84070-6-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-6-RR (CD38 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 84070-6-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (84070-6-RR, Clone: 241246D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84070-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84070-6-RR against Human CD38 were performed. The affinity constant is 1.11 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Jurkat-Zellen, MOLT-4-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84070-6-RR bindet in WB, IHC, IF/ICC, ELISA CD38 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD38 molecule
• Berechnetes Molekulargewicht: 300 aa, 34 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC007964
• Gene symbol: CD38
• Gene ID (NCBI): 952
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).