CD39/ENTPD1 Monoklonaler Antikörper

CD39/ENTPD1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D2H6

Kat-Nr. : 60323-1-PBS

Synonyme

ENTPD1, 2D2H6, ATP diphosphohydrolase, ATPDase, ATP-DPH

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  WB analysis using 60323-1-Ig (same clone as 60323-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60323-1-Ig (same clone as 60323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60323-1-Ig (CD39/ENTPD1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60323-1-Ig (same clone as 60323-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60323-1-Ig (ENTPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60323-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60323-1-PBS bindet in WB, IHC, IF/ICC, ELISA CD39/ENTPD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD39/ENTPD1 fusion protein Ag6165
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 1
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC047664
• Gene symbol: CD39
• Gene ID (NCBI): 953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing. The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 60323-1-Ig can recognize the soluble form of CD39 (38-478aa) molecule.